Joined Sep 2012
362 Posts | 0+
Clear Lake, Iowa
Effective June 2, 2013
Appendix 2: Scrapie Disinfection Guidelines
The following suggested procedures do not guarantee total and complete disinfection and inactivation of the infectious agent. Nonetheless, current information regarding the efficiency of following these disinfection procedures under laboratory conditions suggests these procedures will reduce infectivity in the environment. Until more specific information is available, good sanitary practices are recommended following each lambing. The following methods below should be applied to lambing areas where infected or exposed animals have lambed.
Pastures
1. If practical, till soil under or do not use area to graze susceptible animals.
2. If this is impractical, do not use the pasture until the animal waste has decomposed and the weather has had an opportunity to dilute any infectivity.
Drylots
1. Remove the manure and bedding and, when practical, the top 1 to 2 inches of soil to reduce contamination.
2. Bury or till under the removed material; or, compost the removed material in areas not accessed by domestic or wild ruminants until it can be buried or tilled under.
Earth Surfaces Inside Structures or Used for Confined Lambing Pens
1. Remove the organic material and, when practical, the top 1 to 2 inches of soil to reduce contamination.
2. Bury or till under the removed material; or, compost the removed material in areas not accessed by domestic or wild ruminants until it can be buried or tilled under.
Non-earth Surfaces
(These include cement, wood, metal, tools, equipment, instruments, feed, hay, bedding, and other materials.)
1. Remove all organic material. Bury, incinerate, or compost the removed material in areas not accessed by domestic or wild ruminants and then till under, bury or incinerate.
2. When practical for other items bury or incinerate by high-temperature incineration methods.
3. Clean and wash surfaces and remaining items using hot water and detergent. Allow all surfaces, tools, and equipment to dry completely before disinfecting and sanitizing using the following suggested methods. a. Autoclave instruments, small tools, and other items at 277 °F for 1 hour. This method is more effective when preceded by the treatment described in b or c below.
For General Distribution 66
Scrapie Program Standards Volume 2 March 2013
b. To clean dry surfaces, apply a 2 percent available chlorine solution1 (equivalent to about 20,000 p/m; available chlorine: 50 ounces. [6-1/4 cups] bleach to enough water (78 ounces. or 9 . cups) to give 1 gallon of solution) at room temperature (at least 65 °F) for 1hour. Note: Bleach is caustic and can be hazardous if swallowed, gets in the eyes, is breathed in, or is left on the skin. Further, care must be taken to prevent contamination of water from run off and to comply with any environmental regulations for use of this product. Read the material safety data sheet prior to use and use appropriate personal protective equipment or hire trained personnel to do the work.
c. To clean dry surfaces, apply 1-molar solution of sodium hydroxide1 (approximately 4-percent solution [5 ounces sodium hydroxide dissolved in l gallon water]) at room temperature (at least 65 °F) for at least 1 hour. Synonyms for sodium hydroxide are caustic soda, soda lye, and sodium hydrate. Note: Sodium hydroxide is caustic and can be hazardous if swallowed, gets in the eyes, is breathed in, or is left on the skin. Further, care must be taken to prevent contamination of water from run off and to comply with any environmental regulations for use of this product. Read the material safety data sheet prior to use and use appropriate personal protective equipment or hire trained personnel to do the work.
1 40 CFR § 152 declares prions a pest under the Federal Insecticide, Fungicide and Rodenticide Act (FIFRA). Accordingly, only products registered with the Environmental Protection Agency (EPA) specifically for the reduction of prion infectivity can be used at these sites. Currently there are no EPA registered products available; EPA has therefore granted APHIS an exemption for the use of chlorine and sodium hydroxide for use in its prion control and eradication programs. The instructions above conform to those on the exemption labels.
For General Distribution
Appendix 2: Scrapie Disinfection Guidelines
The following suggested procedures do not guarantee total and complete disinfection and inactivation of the infectious agent. Nonetheless, current information regarding the efficiency of following these disinfection procedures under laboratory conditions suggests these procedures will reduce infectivity in the environment. Until more specific information is available, good sanitary practices are recommended following each lambing. The following methods below should be applied to lambing areas where infected or exposed animals have lambed.
Pastures
1. If practical, till soil under or do not use area to graze susceptible animals.
2. If this is impractical, do not use the pasture until the animal waste has decomposed and the weather has had an opportunity to dilute any infectivity.
Drylots
1. Remove the manure and bedding and, when practical, the top 1 to 2 inches of soil to reduce contamination.
2. Bury or till under the removed material; or, compost the removed material in areas not accessed by domestic or wild ruminants until it can be buried or tilled under.
Earth Surfaces Inside Structures or Used for Confined Lambing Pens
1. Remove the organic material and, when practical, the top 1 to 2 inches of soil to reduce contamination.
2. Bury or till under the removed material; or, compost the removed material in areas not accessed by domestic or wild ruminants until it can be buried or tilled under.
Non-earth Surfaces
(These include cement, wood, metal, tools, equipment, instruments, feed, hay, bedding, and other materials.)
1. Remove all organic material. Bury, incinerate, or compost the removed material in areas not accessed by domestic or wild ruminants and then till under, bury or incinerate.
2. When practical for other items bury or incinerate by high-temperature incineration methods.
3. Clean and wash surfaces and remaining items using hot water and detergent. Allow all surfaces, tools, and equipment to dry completely before disinfecting and sanitizing using the following suggested methods. a. Autoclave instruments, small tools, and other items at 277 °F for 1 hour. This method is more effective when preceded by the treatment described in b or c below.
For General Distribution 66
Scrapie Program Standards Volume 2 March 2013
b. To clean dry surfaces, apply a 2 percent available chlorine solution1 (equivalent to about 20,000 p/m; available chlorine: 50 ounces. [6-1/4 cups] bleach to enough water (78 ounces. or 9 . cups) to give 1 gallon of solution) at room temperature (at least 65 °F) for 1hour. Note: Bleach is caustic and can be hazardous if swallowed, gets in the eyes, is breathed in, or is left on the skin. Further, care must be taken to prevent contamination of water from run off and to comply with any environmental regulations for use of this product. Read the material safety data sheet prior to use and use appropriate personal protective equipment or hire trained personnel to do the work.
c. To clean dry surfaces, apply 1-molar solution of sodium hydroxide1 (approximately 4-percent solution [5 ounces sodium hydroxide dissolved in l gallon water]) at room temperature (at least 65 °F) for at least 1 hour. Synonyms for sodium hydroxide are caustic soda, soda lye, and sodium hydrate. Note: Sodium hydroxide is caustic and can be hazardous if swallowed, gets in the eyes, is breathed in, or is left on the skin. Further, care must be taken to prevent contamination of water from run off and to comply with any environmental regulations for use of this product. Read the material safety data sheet prior to use and use appropriate personal protective equipment or hire trained personnel to do the work.
1 40 CFR § 152 declares prions a pest under the Federal Insecticide, Fungicide and Rodenticide Act (FIFRA). Accordingly, only products registered with the Environmental Protection Agency (EPA) specifically for the reduction of prion infectivity can be used at these sites. Currently there are no EPA registered products available; EPA has therefore granted APHIS an exemption for the use of chlorine and sodium hydroxide for use in its prion control and eradication programs. The instructions above conform to those on the exemption labels.
For General Distribution